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Any effort to stretch, mash, or strengthen wont change your IT band (ref)

Consistent Specifications Standardized 10mL glass vials built to predictable research specifications for qualified laboratory professionals

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Often, multiple samples with different levels of dilution will be cultured so at least one of them will fit this ideal range

Generally well tolerated in human studies

Importantly, the ability of the model to recover barrier integrity after transient permeability, is essential to provide a prototype for studying gut maturation overtime, particularly when assessing dietary (bioactive) components for their ability to accelerate this transition